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Sonic Hedgehog (SHh)

Cat. #: SHH

Cat.# Description Size Quantity Price

SHH-005 sonic Hedgehog, human 5 µg $235.00

SHH-025 sonic Hedgehog, human 25 µg $995.00

SHH-100 sonic Hedgehog, human 100 µg $3,795.00


Product Data Sheet (PDF)

The vertebrate homologs of Hh comprise of Sonic (SHh), Desert (DHh) and Indian (IHh) hedgehogs.  They play important roles in embryonic development and in diseases such as cancer.  Human SHh precursor is a 45 kDa protein.  An autocatalytic reaction yields a 19 kDa N-terminal domain that is biologically active.

Because of unique post-translational modifications, SHh produced in mammalian cells is much more active and, importantly, more biologically authentic than SHh produced from E. coli. StemRD expresses and purifies human SHh from a human cell line in animal-free medium.  This method avoids potential artificial effect caused by bacterial product known to present in commercial SHh made from E. coli (1).  Through a novel and proprietary process, the protein is purified to near homogeneity. Data shown below is from a typical experiment when compared to an E. coli product on the market.

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 Fig. 1. SHh from StemRD made in human cells is 10-50 times more active than a commercial product made in E. coli. Activity is detected by a Gli reporter gene assay in Swiss 3T3 cells.

References:

1. Wakelin et al. Commercially available recombinant sonic hedgehog up-regulates Ptc and modulates the cytokine and chemokine expression of human macrophages: an effect mediated by endotoxin contamination? Immunobiology. 2008;213(1):25-38.



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