“Endosome Escaping”
Cell Penetrating Peptides

The low efficiency of using recombinant proteins to generate iPS cells is partly due to the entrapment of the transduced proteins in endosomes. One solution to this problem is the use of “endosome escaping” cell penetrating peptides. StemRD now offers a number of such peptides that may help improve iPS efficiency.

TAT-HA2 : TAT fused to HA2 peptide at C terminus
HA2-TAT : TAT fused to HA2 peptide at N terminus

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References:

1. El-Sayed A, Futaki S, Harashima H. Delivery of macromolecules using arginine-rich cell-penetrating peptides: ways to overcome endosomal entrapment. AAPS J. 2009; 11:13
2. Sugita T, et al. Improved cytosolic translocation and tumor-killing activity of Tat-shepherdin conjugates mediated by co-treatment with Tat-fused endosome-disruptive HA2 peptide. BBRC, 2007;363:1027
   
3. Wadia JS, Stan RV, Dowdy SF. Transducible TAT-HA fusogenic peptide enhances escape of TAT-fusion proteins after lipid raft macropinocytosis. Nature Medicine. 2004; 10:310