|Reconstitution||Before reconstitution, we recommend a brief spin to drive down any material dislodged from the bottom of the tube. The compound is soluble in DMSO.|
|Stability||The powder is stable for at least 2 year if stored at -20 °C. The dissolved compound is stable for at least 1 month at 4 °C, but should be stored in aliquots at -20 °C for longer term. Protect from light.
|Purity||Greater than 99% as determined by LC/MS analysis. LC/MS and/or NMR data available upon request.|
|Biological Activity||In a cell-based assay measuring the activation of the Gli-1 reporter gene, this compound gives IC50 of 5-20 nM.|
|Country of Origin||USA|
GDC-0449 (Vismodegib) is potent antagonist of SMO (smoothened). This small molecule compound is a hedgehog pathway inhibitor by directly binding to SMO. This molecule has been developed by Genentech for clinical application against hedgehog-dependent cancers.
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van Hoff DD, et al. N Engl J Med. Sep 17, 2009 361: 1164-72;
Rudin CM et al. N Engl J Med. 2009 Sep 17; 361(12):1173-8.
BCR-ABL-positive leukemia cells. Stem Cells Dev. 2012 Nov 1;21(16):2939-48.