| Source | M.W. | 25 kDa | CAS No. | ||
|---|---|---|---|---|---|
| Structural Info | |||||
| Formulation | Lyophilized in sterile filtered solution of acetonitrile/TFA and PBS | ||||
| Reconstitution | Before reconstitution, we recommend a brief spin to drive down any material dislodged from the bottom of the tube. The lyophilized protein should be reconstituted in 5 mM HCl. Further dilutions should be made in buffer or medium containing carrier proteins, such as albumin or serum. |
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| Stability | The lyophilized protein is stable for at least 1 year if stored at -80 °C. Reconstituted protein is stable for at least 1 month at 4 °C, but should be stored in aliquots at -80 °C for longer term. Avoid repeated freeze and thaw. |
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| Purity | Greater than 95% as determined by SDS-PAGE and HPLC analyses | ||||
| Biological Activity | The activity was determined by using a SMAD reporter gene assay in human 293 cells. The EC50 ranges from 0.1 – 1.0 ng/ml. | ||||
| Country of Origin | USA | ||||
One of the three closely-related members of the TGF-β family. Human TGF-β1 cDNA encodes a 390 aa precursor with a 29 aa signal peptide. A furin-like convertase cleaves the precursor to form a 249 aa latency-associated peptide (LAP) and a C-terminal 112 aa mature TGF-β1. Disulfide-linked mature TGF-beta1 is dissociated from the LAP upon activation.
StemRD, produces active TGF-β1 in human 293 cells in Xeno-free medium. After a series of chromatography, the protein is purified to near homogeneity. Activity is determined by using a SMAD reporter gene assay with EC50 0.1 – 1.0 ng/mL.
HPLC and SDS-PAGE profiles of TGF-β1, human from StemRD Inc
tgfb, tgf b, tgf beta
tgfb, tgf b, tgf beta
Eyckmans J, Lin GL, Chen CS. Adhesive and mechanical regulation of mesenchymal stem Cell differentiation in human bone marrow andperiosteum-derived progenitor cells. Biol Open. 2012 Nov 15;1(11):1058-68.
http://www.ncbi.nlm.nih.gov/pubmed/23213385
http://www.ncbi.nlm.nih.gov/pubmed/23213385